| Description | Pepstatin Ammonium is a specific inhibitor of aspartic proteaseproduced by actinomycetes(hemoglobin-pepsin, hemoglobin-proctase, casein-pepsin, casein-proctase, casein-acid protease and hemoglobin-acid protease with IC50s of 4.5 nM, 6.2 nM, 150 nM, 290 nM, 520 nM and 260 nM , respectively). Pepstatin Ammonium also inhibits HIV protease. |
| In vitro | Pepstatin inhibits the recombinant HIV protease with an IC50 of 250 μM. |
| In vivo | Pepstatin suppresses stomach ulceration of the pylorus in ligated Shay rats[1]. |
| Target activity | Hemoglobin-proctase:6.2 nM , Hemoglobin-acid protease:260 nM , Hemoglobin-pepsin:4.5 nM , Casein-pepsin:150 nM , Casein-acid protease:520 nM , Casein-proctase:290 nM |
| Synonyms | Pepstatin A Ammonium |
| molecular weight | 702.92 |
| Molecular formula | C34H66N6O9 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 41.67 mg/mL (59.28 mM), Sonication is recommended. |
| References | 1. Umezawa H, et al. Pepstatin, a new pepsin inhibitor produced by Actinomycetes. J Antibiot (Tokyo). 1970 May;23(5):259-62. 2. Seelmeier S, et al. Human immunodeficiency virus has an aspartic-type protease that can be inhibited by pepstatin A. Proc Natl Acad Sci U S A. 1988 Sep;85(18):6612-6. |