| Description | PEG400 (Polyethylene glycol 400) is a polymer formed from repeating units of ethylene glycol that is water soluble, low immunogenicity, and biocompatible. PEG400 is a neutral polymer with a molecular weight of 400. |
| In vitro | 方法: 人结直肠腺癌细胞 Caco-2 用 PEG400 (30 w/v% in 100 μL) 处理 30 min，使用 MTT 方法检测细胞生长抑制情况。结果: PEG400 处理降低了细胞活力，与对照组相比，细胞活力为 42%±2%。[1] |
| In vivo | 方法: 为研究体内活性，将 Simvastatin (60 mg/kg，aqueous 2% DMSO+30% PEG 400+5% Tween 80) 灌胃给药给 C57BL/6J 小鼠，每天一次，持续六周，给予 CF 饮食。结果: Simvastatin 治疗使血清胆固醇水平降低了 18%，视网膜胆固醇和脂蛋白胆固醇含量分别降低了 24% 和 21%。[2]方法: 为研究 Rapamycin 寿命的影响，将 Rapamycin (8 mg/kg in DMSO+5% PEG-400+5% Tween-80) 腹腔注射给 20–21 个月大的 C57BL/6J 小鼠，每天一次，持续三个月。结果: Rapamycin 治疗 3 个月足以将中年小鼠的预期寿命提高60%，并改善其健康寿命。[3] |
| molecular weight | N/A |
| Molecular formula | HO(CH2CH2O)nH |
| Storage | store at 4°C | Shipping with blue ice. |
| Solubility | DMSO: 100 mg/mL, Sonication is recommended. H2O: 100 mg/mL, Sonication is recommended. |
| References | 1. Pham Le Khanh H, et al. Comparative Investigation of Cellular Effects of Polyethylene Glycol (PEG) Derivatives. Polymers (Basel). 2022 Jan 11;14(2):279. 2. Mast N, et al. Retinal Cholesterol Content Is Reduced in Simvastatin-Treated Mice Due to Inhibited Local Biosynthesis Albeit Increased Uptake of Serum Cholesterol. Drug Metab Dispos. 2018 Nov;46(11):1528-1537. 3. Bitto A, et al. Transient rapamycin treatment can increase lifespan and healthspan in middle-aged mice. Elife. 2016 Aug 23;5:e16351. |