| Description | NucPE1, a nuclear-localized fluorescent hydrogen peroxide, is specifically localized to cellular nuclei without appended targeting moieties. |
| In vitro | NucPE1 features two major visible region absorptions (λabs=468 nm, ε=27,300 M-1cm-1; λabs=490 nm, ε=26,000 /(M·cm)) and a weak emission (λem=530 nm, Φ=0.117). Reaction of NucPE1 with H2O2 triggers a fluorescence increase upon its conversion to fluorophore NucPE1, which possesses one major absorption band at 505 nm (ε=19,100 /(M·cm)) with enhanced emission (λem=530 nm, Φ=0.626). NucPE1 selectively accumulates in the nuclei of a variety of mammalian cell lines as well as in whole model organisms like C. clegans, where it can respond to subcellular changes in H2O2 fluxes. |
| In vivo | NucPE1 imaging reveals a reduction in nuclear H2O2 levels in worms overexpressing sir-2.1 compared to wildtype congeners. |
| Synonyms | Nuclear Peroxy Emerald 1 |
| molecular weight | 483.36 |
| Molecular formula | C29H30BNO5 |
| CAS | 1404091-23-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 25 mg/mL (51.72 mM), Sonication is recommended. |
| References | 1. Stanicka J, et al. NADPH oxidase-generated hydrogen peroxide induces DNA damage in mutant FLT3-expressing leukemia cells. J Biol Chem. 2015 Apr 10;290(15):9348-61. 2. Dickinson BC, et al. A nuclear-localized fluorescent hydrogen peroxide probe for monitoring sirtuin-mediated oxidative stress responses in vivo. Chem Biol. 2011 Aug 26;18(8):943-8. |