| Description | Nucleozin targets influenza A nucleoprotein (NP), a multifunctional, RNA-binding protein necessary for virus replication. |
| In vitro | Nucleozin 抑制了已经进行过核输出的核糖核蛋白(RNPs)在胞质的运输，促进了这些RNPs与细胞内的Rab11形成大型围核聚集体。该作用导致了病毒颗粒数量显著减少，而且往往体积更小。Nucleozin 的主要靶点是病毒的 RNP。通过阻断病母体基因组在胞质的运输，可以有效地抑制 IAV 的复制[1]。 |
| In vivo | Nucleozin通过诱导NP聚集和抑制其在核内积累，阻碍了流感A病毒在体外的复制，并且以nM级的中位有效浓度(EC(50))显示出效果，能够保护小鼠免受致死剂量的禽流感A H5N1的挑战。研究表明，病毒的NP是开发小分子疗法的有效靶点[2]。 |
| Cell experiments | For live imaging, cells were grown in chambered glass bottom dishes and maintained at 37°C in L-15 CO2-independent medium (Gibco) during analysis. Cells were transfected with GFP-NP and infected with PR8 12 h later. For nucleozin treatment, images were acquired for 5 min and then nucleozin was added to a final concentration of 2 μM before imaging for around 20 to 30 min . Images were acquired at 0.25 or 0.71 frame/s and processed with ImageJ[1]. |
| Synonyms | [4-(2-氯-4-硝基苯基)-1-哌嗪基](5-甲基-3-苯基-4-异噁唑基)-甲酮 |
| molecular weight | 426.85 |
| Molecular formula | C21H19ClN4O4 |
| CAS | 341001-38-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 28.66 mg/mL (67.14 mM) |
| References | 1. Amorim M J , Kao R Y , Digard P . Nucleozin Targets Cytoplasmic Trafficking of Viral Ribonucleoprotein-Rab11 Complexes in Influenza A Virus Infection[J]. Journal of Virology, 2013, 87(8):4694-4703. 2. Kao R Y , Yang D , Lau L S , et al. Identification of influenza A nucleoprotein as an antiviral target[J]. Nature Biotechnology, 2010, 28(6):600-605. 3. Zhao, Ming, et al. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature Communications . 12.1 (2021): 1-14. |
| Citations | 1. Zhao, Ming, et al. GCG inhibits SARS-CoV-2 replication by disrupting the liquid phase condensation of its nucleocapsid protein. Nature Communications. 2021 Apr 9;12(1):2114. doi: 10.1038/s41467-021-22297-8. |