| Description | NIH-12848 is a putative inhibitor of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) with an IC50 of 1 μM. |
| In vitro | NIH-12848能够抑制Na+/K+-ATPase在mpkCCD细胞增长至密集时向细胞膜的转运，并且可逆地阻止它们在培养皿上形成‘圆顶’。此外，NIH-12848对PI5P4Kγ的IC50约为1 μM，但在高达100 μM的浓度下不抑制α和β PI5P4K亚型。这些由NIH-12848引起的效应，通过特异性RNAi敲减PI5P4Kγ可以复制，但对PI5P4Ks α或β的敲减则不会。因此，NIH-12848可能是探索PI5P4Ks细胞生理学的一个强大工具。 |
| Target activity | PI5P4Kγ:1 μM |
| molecular weight | 385.41 |
| Molecular formula | C20H14F3N3S |
| CAS | 959551-10-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 55 mg/mL (142.71 mM) |
| References | 1. Clarke, J., Giudici, M., Burke, J., Williams, R., Maloney, D., Marugan, J., & Irvine, R. (2015). The function of phosphatidylinositol 5-phosphate 4-kinase γ (PI5P4Kγ) explored using a specific inhibitor that targets the PI5P-binding site. Biochemical Journal, 466(2), 359-367. doi: 10.1042/bj20141333 |