| Description | Neophytadiene, a diterpene discovered in Turbinaria ornate, showcases desirable bioactive properties including anti-inflammatory, antioxidant, and cardioprotective effects. |
| In vitro | Neophytadiene has cytotoxic on RAW 264.7 cells with an IC 50 of 50 μM[1]. Neophytadiene (50-100 μM; 30 min) successfully inhibits the NO production level and downregulates inducible nitric oxide synthase (iNOS) production in LPS (1 μg/mL)-induced RAW264.7 cells[1]. Neophytadiene (25-100 μM; 22 hours) significantly inhibits the NO production and inflammatory cytokines TNF-α, IL-6 and IL-10 and IL-10 in LPS (1 μg/mL)-induced RAW264.7 cells[1]. |
| In vivo | Neophytadiene (50 mg/kg; p.o.; daily; for 7 days) shows dose-dependent reduction in the alanine aminotransferase (ALT) level[1]. Neophytadiene (25-50 mg/kg; p.o.; daily; for 7 days) significantly reduces LPS (10 mg/kg)-increased IL-6 and 1L-10 levels and in heart tissue[1]. Neophytadiene (25-50 mg/kg; p.o.; daily; for 7 days) significantly reduces LPS (10 mg/kg)-increased PGE2 mRNA expression in heart tissue[1]. Neophytadiene significantly inhibits LPS (10 mg/kg)-induced NO production and inflammatory cytokines TNF-α, IL-6 and IL-10 and IL-10 in vivo[1]. Animal Model: Adult male Sprague Dawley rats (6-8 weeks)[1]Dosage: 12 mg/kg, 25 mg/kg, 50 mg/kg Administration: Oral administration, daily, for 7 days Result: Decreased haemoglobin (HGB) level, ALT level, and heart tissue TNF-α, IL1β, NF-κB, iNOS, PI3k/AktandMAPK in LPS (10 mg/kg)-treat rats. |
| molecular weight | 278.52 |
| Molecular formula | C20H38 |
| CAS | 504-96-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Meenakshi Bhardwaj, et al. Neophytadiene from Turbinaria ornata Suppresses LPS-Induced Inflammatory Response in RAW 264.7 Macrophages and Sprague Dawley Rats. Inflammation. 2020 Jun;43(3):937-950. |