| Description | Neomycin sulfate (Framycin sulfate) is a broad-spectrum aminoglycoside antibiotic that blocks the synthesis of bacterial proteins in order to exert antimicrobial activity. Neomycin sulfate is commonly used to screen prokaryotic and eukaryotic cells for Neo resistance genes. |
| In vitro | 方法：BHK-21 细胞、VERO 细胞和 FEA 细胞用 Neomycin sulfate (1000-20000 μg/mL) 处理 24 h，使用 MTT 方法检测细胞活力。结果：9000-20000 μg/mL Neomycin 处理后，BHK-21 细胞的活力显着降低。3000 μg/mL Neomycin 处理时，FEA 细胞活力显着降低。20000 μg/mL Neomycin 处理时，VERO 细胞活力不受影响。[1]方法：小鼠耳蜗毛细胞 HEI-OC-1 用 Neomycin sulfate (2 mM) 处理 24 h，使用 Flow Cytometry 检测细胞凋亡情况。结果：20 μM GM1 显著降低了 Neomycin sulfate 诱导的 HEI-OC-1 细胞的死亡。[2] |
| In vivo | 方法：为检测体内活性，将 Neomycin sulfate (20 mg/mouse) 和 bacitracin (20 mg/mouse) 灌胃给药给 C57BL/10 小鼠，每天一次，持续七天。结果：Neomycin 和 bacitracin 联合使用可降低肠通透性，增加回肠中 ZO-1、JAM-A 和 occludin 以及结肠中 ZO-1、claudin-3 和 claudin-4 的基因表达。[3]方法：为耗尽小鼠的肠道微生物群，将抗生素 (ABX) vancomycin (0.5 g/L)、ampicillin (1 g/L)、 Neomycin sulfate (1 g/L) 和 metronidazole (1 g/L) 饮水给药给小鼠，持续两周。结果：抗生素明显降低了肠道微生物群的多样性和组成。[4] |
| Synonyms | Framycin sulfate, 硫酸新霉素 |
| molecular weight | 908.87 |
| Molecular formula | C23H46N6O13·3H2SO4 |
| CAS | 1405-10-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: Insoluble H2O: 10 mM |
| References | 1. Kovacik A, et al. Cytotoxic effect of aminoglycoside antibiotics on the mammalian cell lines. J Environ Sci Health A Tox Hazard Subst Environ Eng. 2021;56(1):1-8. 2. Li Y, et al. The Ganglioside Monosialotetrahexosylganglioside Protects Auditory Hair Cells Against Neomycin-Induced Cytotoxicity Through Mitochondrial Antioxidation: An in vitro Study. Front Cell Neurosci. 2021 Sep 27;15:751867. 3. Nevado R, et al. Neomycin and bacitracin reduce the intestinal permeability in mice and increase the expression of some tight-junction proteins. Rev Esp Enferm Dig. 2015 Nov;107(11):672-6. 4. Li F, et al. The intestinal microbial metabolite nicotinamide n-oxide prevents herpes simplex encephalitis via activating mitophagy in microglia. Gut Microbes. 2022 Jan-Dec;14(1):2096989. |
| Citations | 1. Shan T, Huang Y, Zhao Z, et al. Ketogenic diet restrains herpes simplex encephalitis via gut microbes. Microbes and Infection. 2022: 105061. 2. Li F, Wang Y, Song X, et al. The intestinal microbial metabolite nicotinamide n-oxide prevents herpes simplex encephalitis via activating mitophagy in microglia. Gut Microbes. 2022, 14(1): 2096989 3. Zhang Z, Ye J, Liu X, et al.Huangqi Guizhi Wuwu decoction alleviates oxaliplatin-induced peripheral neuropathy via the gut-peripheral nerve axis.Chinese Medicine.2023, 18(1): 1-15. |