| Description | Neohesperidin Dihydrochalcone (Neohesperidin DC) is an artificial sweetener derived from citrus. |
| In vivo | Neohesperidin dihydrochalcone administration causes a significant reduction in the activities of two useful markers of liver damage, AST and ALT. The relative levels of NF-κB, IL-6, IL-1β and TNF-α protein in the liver of PQ-treated mice are inhibited by neohesperidin dihydrochalcone[3]. The embryotoxicity/teratogenicity of neohesperidin dihydrochalcone is examined in Wistar Crl:(WI)WU BR rats. No adverse effects are observed at neohesperidin dihydrochalcone levels of up to 5% of the diet, the highest dose level tested, at which the rats consumed about 3.3 g/kg body weight/day[4]. |
| Cell experiments | WST-8 dye is used in the cell viability assay. HIT-T15 and HUVEC cells are grown and maintained in Dulbecco's modified Eagle's medium, supplemented with 10% fetal bovine calf serum. 1000 cells in each well are incubated with various concentrations of neohesperidin dihydrochalcone (50, 100, 500 μM, 1 mM) and other compounds. After treating HIT-T15 and HUVEC cells with 500 μM HOCl, WST-8 dye is added to each well, and the absorbance is detected at 420 nm with microplate reader[1]. |
| Synonyms | NHDC, 新橙皮苷二氢查尔酮, 新橙皮苷二氢查耳酮, Neohesperidin DC, NCI-c60764 |
| molecular weight | 612.58 |
| Molecular formula | C28H36O15 |
| CAS | 20702-77-6 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | Ethanol: 100 mg/mL (163.24 mM) DMSO: 50 mg/mL (81.62 mM) H2O: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Kroeze JH, Chem Senses, 2000, 25(5), 555-559. 2. Winnig M, et al. BMC Struct Biol, 2007, 7, 66. 3. Shi Q, et al. Artificial sweetener neohesperidin dihydrochalcone showed antioxidative, anti-inflammatory and anti-apoptosis effects against paraquat-induced liver injury in mice. Int Immunopharmacol. 2015 Dec;29(2):722-9. 4. Waalkens-Berendsen DH, et al. Embryotoxicity and teratogenicity study with neohesperidin dihydrochalcone in rats. Regul Toxicol Pharmacol. 2004 Aug;40(1):74-9. |