| Description | NBD-F (4-Fluoro-7-nitrobenzofurazan) is a fluorescent derivatization compound for amino acid analysis. |
| In vitro | 随着有机相百分比的变化，NBD-F的保留时间相对稳定，而衍生物的保留时间发生变化。流动相的pH值影响NBD-F与衍生物的分离[1]。NBD-F最近被应用于其他氨基酸衍生物如N-甲基-D-天冬氨酸和谷胱甘肽的分析。NBD-F的使用具有几个优点，即衍生化程序简单，其衍生物具有很高的稳定性[2]。 |
| Synonyms | 4-Fluoro-7-nitrobenzofurazan |
| molecular weight | 183.1 |
| Molecular formula | C6H2FN3O3 |
| CAS | 29270-56-2 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 110 mg/mL (600.76 mM) |
| References | 1. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62. 2. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304. |