| Description | MTP 131 (acetate) is a small mitochondrially-targeted tetrapeptide. |
| In vitro | 细胞预处理MTP 131 (acetate)能够抑制H2O2引起的细胞毒性，并以剂量依赖性方式减少LDH释放，相比仅用H2O2处理的细胞。MTP 131 (acetate)预处理也防止了线粒体去极化和ROS生成。此外，MTP 131 (acetate)预处理抑制了细胞色素c从线粒体释放至细胞质，并显著减少了与仅H2O2处理相比的RGC-5细胞凋亡[1]。 |
| In vivo | 18周龄的db/db小鼠，通过二氢乙菲啶氧化测量显示，其肾脏和心脏的超氧化物水平有所下降，同时尿白蛋白水平、系膜基质累积及尿液中H2O2水平有所上升。MTP 131 acetate的施用显著抑制了db/db小鼠中尿白蛋白、尿液H2O2及系膜基质累积的增加，并完全保持了这些小鼠肾脏超氧化物产生的水平。此外，MTP 131 acetate还降低了db/db小鼠总肾脏溶血卡磷脂及其主要亚型水平，并保持了溶血卡磷脂酰基转移酶1的表达[1]。 |
| molecular weight | 699.84 |
| Molecular formula | C34H53N9O7 |
| CAS | 1334953-95-5 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | PBS (pH 7.2): 10 mg/mL |
| References | 1. Chen M, et al. Protective effect of mitochondria‑targeted peptide MTP‑131 against oxidative stress‑induced apoptosis in RGC‑5 cells. Mol Med Rep. 2017 Apr;15(4):2179-2185. 2. Miyamoto S, Zhang G, Hall D, Oates PJ, Maity S, Madesh M, Han X, Sharma K. Restoring mitochondrial superoxide levels with elamipretide (MTP-131) protects db/db mice against progression of diabetic kidney disease. J Biol Chem. 2020 May 22;295(21):7249-7260. doi: 10.1074/jbc.RA119.011110. Epub 2020 Apr 10. PMID: 32277051; PMCID: PMC7247302. 3. Miyamoto, S., Zhang, G., Hall, D., et al.Restoring mitochondrial superoxide levels with elamipretide (MTP-131) protects db/db mice against progression of diabetic kidney diseaseJ. Biol. Chem.295(21)7249-7260(2020) |