| Description | Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) is a truncated peptide derived from the human Myelin Oligodendrocyte Glycoprotein (MOG). |
| In vitro | CD8CD3和CD4CD3细胞经过CD8/CD4珠子富集后，分别占总CD3细胞的86%和94%。这些细胞被MOG35-55肽激活，并应用于增殖实验。尽管CD8 T细胞对MOG35-55的反应通常低于CD4 T细胞，但CD8 T细胞的反应并不总是依赖于CD4。与CD4 T细胞相比，CD8 T细胞分泌的IFN-γ和IL-4较少[1]。 |
| In vivo | 采用野生型B6幼稚小鼠，通过将MOG35-55特异性T细胞从主动诱导EAE(aEAE)的B6小鼠中采集并移植至其体内，从而在这些小鼠中诱发了EAE。研究发现，与移植CD4 T细胞相比，从免疫B6小鼠中转移CD8 T细胞至幼稚B6小鼠后，后者显示出了类似的EAE评分以及轻微的炎症和脱髓鞘现象[1]。 |
| Animal experiments | Female C57BL/6 mice (n?=?20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide.?At 14 days after immunization, T cells were isolated from the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis.?These cells were stimulated by MOG35-55 peptide and applied to proliferation assays.?The interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA).?For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells.?EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining[1]. |
| Synonyms | 髓鞘少突胶质细胞糖蛋白, MOG (35-55) |
| molecular weight | 2581.95 |
| Molecular formula | C118H177N35O29S |
| CAS | 149635-73-4 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: Insoluble DMSO: Insoluble |
| References | 1. Peng Y, Zhu FZ, Chen ZX,et al.Characterization of myelin oligodendrocyte glycoprotein (MOG)35-55-specific CD8+ T cells in experimental autoimmune encephalomyelitis[J].Chin Med J (Engl). 2019 Dec 20;132(24):2934-2940. 2. Ichikawa M , , Johns T G , Liu J , , et al. Analysis of the fine B cell specificity during the chronic/relapsing course of a multiple sclerosis-like disease in Lewis rats injected with the encephalitogenic myelin oligodendrocyte glycoprotein peptide 35-55[J]. Journal of Immunology, 1996, 157(2):919. |