| Description | ML67-33 rapidly and reversibly affects K2P2.1 (TREK-1) (EC50s: 36.3 μM and 9.7 μM in cell-free and HEK293 cells, respectively). ML67-33 is a selective activator of temperature- and mechano-sensitive K2P channels. |
| In vitro | ML67-33 activates K2P channels and mutants. It has an EC50s of 21.8±1.3 μM, 49.4±1.1 μM, 30.2±1.4 μM, and 27.3±1.2 μM for K2P2.1 (TREK-1) W275S, K2P2.1 (TREK-1)-3G, K2P10.1 (TREK-2), K2P4.1 (TRAAK), respectively. |
| Target activity | K2P2.1 (TREK-1, HEK293 cells):9.7 μM(EC50), K2P2.1 (TREK-1):(EC50)36.3 μM |
| molecular weight | 374.27 |
| Molecular formula | C18H17Cl2N5 |
| CAS | 1443290-89-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Bagriantsev SN, et al. A high-throughput functional screen identifies small molecule regulators of temperature- and mechano-sensitive K |