| Description | Mito-TEMPO is a mitochondria-targeted superoxide dismutase mimetic. Mito-TEMPO scavenges superoxide and alkyl radicals and prevents mitochondrial oxidation, necrosis and apoptosis. |
| In vitro | 方法：人神经母细胞瘤细胞 SH-SY5Y 用 Mito-TEMPO (25-100 μM) 处理 24 h，使用 MTT assay 检测细胞活力。结果：Mito-TEMPO 处理组对细胞没有显示出细胞毒性作用，Mito-TEMPO 处理后检测到细胞活力的显著增加。[1]方法：正常大鼠近端肾小管上皮细胞系 NRK-52E 用 Mito-TEMPO (10 μM) 预处理 1 h，再用 oxalate (700 μM) 刺激 1 h，使用 MMP assay kit (JC-1) 检测线粒体膜电位。结果：对照组细胞呈现明亮的红色荧光。与对照组相比，oxalate 处理减弱了红色荧光，用 Mito-TEMPO 预处理逆转了这些变化。结果表明，oxalate 诱导线粒体功能障碍，Mito-TEMPO 可抑制这种作用。[2] |
| In vivo | 方法：为研究对肝毒性的保护作用，将 APAP (300 mg/kg) 腹腔注射给 C57BL/6J 小鼠，1.5-3 h 后腹腔注射 Mito-TEMPO (20 mg/kg in saline)。结果：Mito-TEMPO 对 APAP 的晚期肝毒性有保护作用。[3]方法：为研究对冠状动脉血管舒张和内皮 SK 通道活性的影响，将 Mito-TEMPO (1 mg/kg in saline) 腹腔注射给患有或不患有糖尿病的 C57BL/6J 小鼠，每天一次，持续四周。结果：用 Mito-TEMPO 治疗 4 周后，与未治疗的糖尿病小鼠相比，糖尿病小鼠对 ADP 或 NS309 的冠状动脉内皮依赖性舒张反应和内皮 SK 通道电流显著改善。[4] |
| molecular weight | 510.03 |
| Molecular formula | C29H35N2O2P.Cl |
| CAS | 1334850-99-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 60 mg/mL (117.64 mM), Sonication is recommended. DMSO: 45 mg/mL (88.23 mM), Sonication is recommended. |
| References | 1. Mukem S, et al. Mito-Tempo suppresses autophagic flux via the PI3K/Akt/mTOR signaling pathway in neuroblastoma SH-SY5Y cells. Heliyon. 2021 Jun 15;7(6):e07310. 2. Zhang J, et al. MitoTEMPO Prevents Oxalate Induced Injury in NRK-52E Cells via Inhibiting Mitochondrial Dysfunction and Modulating Oxidative Stress. Oxid Med Cell Longev. 2017;2017:7528090. 3. Du K, et al. Mito-tempo protects against acute liver injury but induces limited secondary apoptosis during the late phase of acetaminophen hepatotoxicity. Arch Toxicol. 2019 Jan;93(1):163-178. 4. Xing H, et al. Chronic Inhibition of mROS Protects Against Coronary Endothelial Dysfunction in Mice With Diabetes. Front Cell Dev Biol. 2021 Feb 18;9:643810. |
| Citations | 1. Zhao X, Miao G, Zhang L, et al. Chlamydia pneumoniae Infection Induces Vascular Smooth Muscle Cell Migration and Atherosclerosis Through Mitochondrial Reactive Oxygen Species-Mediated JunB-Fra-1 Activation. Frontiers in Cell and Developmental Biology. 2022, 10: 879023-879023 2. Tian C, Han X, He L, et al. Transient receptor potential ankyrin 1 contributes to the ATP-elicited oxidative stress and inflammation in THP-1-derived macrophage. Molecular and Cellular Biochemistry. 2020: 1-14 3. Wang Y, He X, Xue M, et al.Germacrone protects renal tubular cells against ferroptotic death and ROS release by re-activating mitophagy in diabetic nephropathy.Free Radical Research.2023 (just-accepted): 1-36. 4. Yan Z, Niu L, Wang S, et al.Intestinal Piezo1 aggravates intestinal barrier dysfunction during sepsis by mediating Ca2+ influx.Journal of Translational Medicine.2024, 22(1): 1-12. 5. Tang X, Zheng N, Lin Q, et al.Hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect neurons from cardiac arrest–induced pyroptosis.Neural Regeneration Research.2025: 10.4103. |