| Description | Methyl-β-cyclodextrin (Methyl-beta-cyclodextrin) is a macrocyclic compound used as a solubilizer for hydrophobic compounds in biological experiments. Methyl-β-cyclodextrin is also a lipid raft inhibitor with cholesterol-lowering activity and potential antitumor activity. |
| In vitro | 方法：五种原发性渗出性淋巴瘤 (PEL) 细胞系 BCBL-1、BC-1、BC-3、TY-1 和 GTO 用 Methyl-β-cyclodextrin (0-10 mM) 处理 24 h，使用 MTT assay 检测细胞活力。结果：Methyl-β-cyclodextrin 剂量依赖性抑制 PEL 细胞生长，IC50 在 3.33-4.23 mM 之间。[1]方法：鸡肝癌细胞 LMH 用 Methyl-β-cyclodextrin (10 mM) 处理 1 h，然后在感染 FAdV-4 之前、期间和之后的不同时间点添加胆固醇 (50 µg/mL)，使用 Western Blot 检测靶点蛋白表达水平。结果：当 LMH 细胞在感染前用增加浓度的 Methyl-β-cyclodextrin 预处理时，penton 蛋白水平以剂量依赖的方式降低。[2] |
| In vivo | 方法：为检测体内抗肿瘤活性，将 Methyl-β-cyclodextrin (500 mg/kg) 腹腔注射给携带 PEL 肿瘤 BCBL-1 的 NRJ 小鼠，每天一次，持续二十一天。结果：Methyl-β-cyclodextrin 显著抑制 PEL 细胞的生长和侵袭，没有明显的不良反应。[1]方法：为研究对胶原蛋白的调节作用，将 Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) 皮内注射给 SKH1 小鼠，每周两次，持续两个月。结果：Methyl-β-cyclodextrin 显示出强大的 COL I 上调活性，导致皮肤厚度增加。[3] |
| Cell experiments | PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined[1]. |
| Target activity | PEL:3.33-4.23 mM. |
| Synonyms | Methyl-beta-cyclodextrin, 甲基-β-环糊精, 甲基倍他环糊精, beta-Cyclodextrin methyl ethers |
| molecular weight | 1310(Average) |
| Molecular formula | C54H94O35 |
| CAS | 128446-36-6 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 55 mg/mL (41.98 mM) |
| References | 1. Gotoh K, et al. The antitumor effects of methyl-β-cyclodextrin against primary effusion lymphoma via the depletion of cholesterol from lipid rafts. Biochem Biophys Res Commun. 2014 Dec 12;455(3-4):285-9. 2. Wang T, et al. Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway. Vet Res. 2023 Mar 14;54(1):24. 3. Takeo T, et al. Methyl-beta-cyclodextrin improves fertilizing ability of C57BL/6 mouse sperm after freezing and thawing by facilitating cholesterol efflux from the cells. Biol Reprod. 2008 Mar;78(3):546-51. 4. Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1. Cell Death & Differentiation. 2019: 1-16. |
| Citations | 1. Lu J Y, Huang W T, Zhou K, et al. Microbial Lipopeptide Supramolecular Self‐Assemblies as a Methuosis‐Like Cell Death Inducer with In Vivo Antitumor Activity. Small. 2021: 2104034. 2. Zhang Y, Xue W, Zhang W, et al. Histone methyltransferase G9a protects against acute liver injury through GSTP1. Cell Death & Differentiation. 2020, 27(4): 1243-1258 3. Li X, Tang H, Huang X, et al. Rigidity‐Dependent Placental Cells Uptake of Silk‐Based Microcapsules. Macromolecular bioscience. 2019: 1900105. 4. Zeng L, Liu H, Liu Z, et al.Defected lipid rafts suppress cavin1-dependent IFN-α signaling endosome in paroxysmal nocturnal hemoglobinuria.International Immunopharmacology.2023, 115: 109468. 5. Wang T, Wang L, Li W, et al.Fowl adenovirus serotype 4 enters leghorn male hepatocellular cells via the clathrin-mediated endocytosis pathway.Veterinary Research.2023, 54(1): 1-14. |