| Description | Methoxychlor, an organochlorine pesticide, is thought to be an endocrine disrupter that affects Ca2 homeostasis and cell viability in different cell models. |
| In vitro | 在HA59T细胞中，methoxychlor通过促进蛋白激酶C敏感的Ca2+可渗透通道入侵，引起[Ca2]i上升，而不引起Ca2从库存释放。Methoxychlor还引发了与[Ca2]i上升无关的凋亡。 |
| Cell experiments | Fura-2, a Ca2?-sensitive fluorescent dye, was applied to measure [Ca2?]i. Methoxychlor at concentrations of 0.1-1 μM caused a [Ca2?]i rise in a concentration-dependent manner. Removal of external Ca2? abolished methoxychlor's effect. Methoxychlor-induced Ca2? influx was confirmed by Mn2?-induced quench of fura-2 fluorescence. Methoxychlor-induced Ca2? entry was inhibited by nifedipine, econazole, SK&F96365, and protein kinase C modulators. Methoxychlor killed cells at concentrations of 10-130 μM in a concentration-dependent fashion. Chelation of cytosolic Ca2? with 1,2-bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid/AM (BAPTA/AM) did not prevent methoxychlor's cytotoxicity. Methoxychlor (10 and 50 μM) induced apoptosis concentration-dependently as determined by using Annexin V/propidium iodide staining |
| molecular weight | 345.65 |
| Molecular formula | C16H15Cl3O2 |
| CAS | 72-43-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 22.5 mg/mL (65.1 mM) |
| References | 1. Horng C T , Chou C T , Tseng H W , et al. Effect of Methoxychlor on Ca²⁺ Homeostasis and Apoptosis in HA59T Human Hepatoma Cells[J]. Chin J Physiol, 2015, 58(1):1-8. |