| Description | ME0328 is a potent and selective PARP inhibitor with IC50 of 0.89 μM for PARP3, about 7-fold selectivity over PARP1. |
| In vitro | ME0328具有溶解性，能渗透细胞，并且在人类肝微粒体和大鼠肝细胞中表现出代谢稳定性。ME0328（10 μM）通过影响A549和MRC5细胞中的ARTD3，显著延迟γH2AX-焦点的解决，而无明显的毒性。[1] |
| Cell experiments | Compound cytotoxicity in A549 and MRC5 cells is evaluated using WST-1 assays. A549 cells are cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin. MRC5 cells are cultured in Minimal Essential Medium supplemented with 10% FCS, penicillin, streptomycin, and l-glutamine. Both cell lines are maintained in a humidified incubator at 37°C and 5% CO2. (Only for Reference) |
| Target activity | PARP3:0.89 μM |
| molecular weight | 321.37 |
| Molecular formula | C19H19N3O2 |
| CAS | 1445251-22-8 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 60 mg/mL (186.7 mM) H2O: < 1 mg/mL (insoluble or slightly soluble) Ethanol: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Lindgren AE, et al. ACS Chem Biol. 2013, 8(8), 1698-1703. |