| In vitro |
In MIN6 cells, in a dose-dependent manner, lactisole inhibited insulin secretion induced by sweeteners, acesulfame-K, sucralose and glycyrrhizin. The IC50 was ～4 mmol/l. Lactisole attenuated the elevation of cytoplasmic Ca2+ concentration ([Ca2+]c) evoked by sucralose and acesulfame-K but did not affect the elevation of intracellular cAMP concentration ([cAMP]c) induced by these sweeteners. Lactisole also inhibited the action of glucose in MIN6 cells. Thus, lactisole significantly reduced elevations of intracellular [NADH] and intracellular [ATP] induced by glucose, and also inhibited glucose-induced insulin secretion[1]. |
| References |
1. Johnson, C., Birch, G.G., and MacDougall, D.B. The effect of the sweetness inhibitor 2(-4-methoxyphenoxy)propanoic acid (sodium salt) (Na-PMP) on the taste of bitter-sweet stimuli. Chem.Senses 19(4), 348-358 (1994). 2. Schiffman, S.S., Booth, B.J., Sattely-Miller, E.A., et al. Selective inhibition of sweetness by the sodium salt of ±2-(4-methoxyphenoxy)propanoic acid. Chem.Senses 24(4), 439-447 (1999). 3. Hamano, K., Nakagawa, Y., Ohtsu, Y., et al. Lactisole inhibits the glucose-sensing receptor T1R3 expressed in mouse pancreatic β-cells. Journal of Endocrinology 226(1), 57-66 (2015). 4. Shim, J., Son, H.J., Kim, Y., et al. Modulation of sweet taste by umami compounds via sweet taste receptor subunit hT1R2. PLoS One 10(4), (2015). 5. Ohtsu, Y., Nakagawa, Y., Nagasawa, M., et al. Diverse signaling systems activated by the sweet taste receptor in human GLP-1-secreting cells. Molecular and Cellular Endocrinology 394(1-2), 70-79 (2014). 6. Sato, S., Hokari, R., Kurihara, C., et al. Dietary lipids and sweeteners regulate glucagon-like peptide-2 secretion. Am. J. Physiol Gastrointest. Liver Physiol. 304(8), 304-308 (2013). |
