| Description | Hygromycin B (Hygrovetine) is an aminoglycoside antibiotic that inhibits protein synthesis by interfering with translocation and causing mistranslation of the 70S ribosome. Hygromycin B can be used to screen prokaryotic or eukaryotic cells transfected with hph or hyg resistance genes. |
| In vitro | 方法：小鼠 PDAC 细胞 14387T 用 lentiCRISPRv2 hygro 慢病毒转染细胞，48 h 后，将转染的细胞在含有 Hygromycin B (500μg/mL) 的新培养基中培养两周，筛选转染成功的细胞。 结果：Puromycin dihydrochloride 筛选出了慢病毒成功转染的细胞。[1]方法：大肠杆菌用含 Hygromycin B (0-8 μg/mL) 的培养基培养，使用 Klett-Summerson colorimeter 监测细胞生长。结果：Hygromycin B 以浓度依赖的方式减少了活细胞的数量并增加了倍增时间。Hygromycin B 在 20 μg/mL 浓度下抑制 50% 的活细胞数，在 25 μg/mL 浓度时生长速率减半。[2] |
| In vivo | 方法：为检测体内抗病毒活性，将 Hygromycin B (0-5 μmoL/kg) 腹腔注射给 MHV-A59 感染的 BALB/c 小鼠，每天两次，持续三天。结果：Hygromycin B 能够在体内降低病毒复制和坏死肝灶的水平。[3] |
| Synonyms | 潮霉素B, Hygrovetine |
| molecular weight | 527.52 |
| Molecular formula | C20H37N3O13 |
| CAS | 31282-04-9 |
| Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 30 mg/mL (56.87 mM) Saline: 527.52 mg/mL (94.78 mM) |
| References | 1. Fan M, et al. UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis. Cell Rep. 2024 Feb 16;43(2):113808. 2. McGaha SM, et al. Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli. Antimicrob Agents Chemother. 2007 Feb;51(2):591-6. 3. Macintyre G, et al. Hygromycin B therapy of a murine coronaviral hepatitis. Antimicrob Agents Chemother. 1991 Oct;35(10):2125-7. 4. Santerre RF, et al. Gene, 1984, 30(1-3), 147-156. 5. Hanif M, et al. Curr Genet, 2002, 41(3), 183-188. |
| Citations | 1. Fan M, Huo S, Guo Y, et al.UDP-glucose dehydrogenase supports autophagy-deficient PDAC growth via increasing hyaluronic acid biosynthesis.Cell Reports.2024, 43(2): 113808. |