| Description | Hydroxystilbamidine ((E)-hydroxystilbamidine), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases. |
| In vitro | Hydroxystilbamidine在使用Nonidet P-40（NP-40）释放多聚体后，允许恢复mRNA。通过对用洗涤剂裂解的细胞核后上清液进行蔗糖梯度分析，用于分析NP-40释放的多聚体的尺寸分布。肝素梯度显示了一些多肋体，而Hydroxystilbamidine梯度则显示出非常重的多肋体的显著大峰。如果在加入NP-40之前存在Hydroxystilbamidine，则这个峰可以可靠地获得[1]。 |
| In vivo | Hydroxystilbamidine在给予绵羊红细胞(SRBC)刺激前作为有效的斑点形成细胞(PFC)反应抑制剂。在整个实验过程中，处理过的小鼠中观察到的PFC数量较少，但随着时间推移，抑制水平逐渐降低。到第14天，Hydroxystilbamidine处理过的小鼠与对照组的PFC数量基本上处于背景水平[2]。 |
| Synonyms | Oxistilbamidinum, 羟芪巴脒, (E)-hydroxystilbamidine, 2-Hydroxy-4,4'-diguanylstilbene, Bis(8-hydroxyquinolinium) sulphate |
| molecular weight | 280.32 |
| Molecular formula | C16H16N4O |
| CAS | 495-99-8 |
| Storage | keep away from direct sunlight|Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: Insoluble |
| References | 1. Lizardi PM. Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine. J Cell Biol. 1980 Oct;87(1):292-6. 2. Folds JD, et al. Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug. Infect Immun. 1975 Mar;11(3):441-4. |