| Description | Hoechst 33258 analog 3 is part of a family of blue fluorescent dyes used to stain DNA. |
| In vitro | Hoechst 33258 和 Hoechst 33342 是一种常用的染料，它们具有相似的激发/发射光谱。这两种染料都可以在大约 350 nm 的紫外光下激发，并在 461 nm 的发射峰值处发出蓝色/青色荧光。未结合的染料在 510-540 nm 范围内具有最大荧光发射。Hoechst 染料能溶于水和有机溶剂，如二甲基甲酰胺或二甲基亚砜，浓度可达 10 mg/mL。其水溶液在 2-6 °C 下避光可稳定存放至少六个月，长期存储时，溶液应在 ≤-20 °C 下冷冻。虽然这些染料能与所有核酸结合，但与富含 AT 的双链 DNA 结合能显著增强荧光。Hoechst 染料可穿透细胞膜，能在活细胞或固定细胞中与 DNA 结合。 |
| molecular weight | 422.52 |
| Molecular formula | C26H26N6 |
| CAS | 23554-98-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 3.57 mg/mL (8.45 mM), Sonication and heating to 70℃ are recommended. |
| References | 1. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. 2. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. 3. a b c "Hoechst Stains". Invitrogren (Molecular Probes). |