| Description | Hoechst 33258 analog 2 is part of a family of blue fluorescent dyes used to stain DNA. |
| In vitro | Hoechst 33258和Hoechst 33342是最常用的染料，它们具有相似的激发/发射光谱。这两种染料在大约350 nm的紫外光下被激发，并在大约461 nm的发射最大值附近发出蓝色/青色荧光。未结合的染料在510-540 nm范围内具有最大荧光发射。Hoechst染料可溶于水和有机溶剂，如二甲基甲酰胺或二甲基亚砜。浓度可达到10 mg/mL。水溶液在2-6 °C下避光保存至少六个月是稳定的。长期存储时，溶液被冻存在≤-20 °C。虽然这些染料可以结合到所有核酸上，但与富含AT的双链DNA结合时，荧光显著增强。Hoechst染料能够渗透细胞并在活细胞或固定细胞中与DNA结合。 |
| molecular weight | 422.52 |
| Molecular formula | C26H26N6 |
| CAS | 23491-54-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: < 0.1 mg/mL (insoluble) DMSO: 60 mg/mL (142.01 mM) |
| References | 1. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505. 2. a b c "Hoechst Stains". Invitrogren (Molecular Probes). 3. Portugal J, Waring MJ. Assignment of DNA binding sites for 4',6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68. |