| Description | HKPerox-2 is an excellently selective and sensitive green fluorescent probe toward H 2 O 2 over 30-fold other tested ROS/RNS in chemical and biological systems. HKPerox-2 is a O-methyl rhodol derivative and specifically recognize H 2 O 2 based on a tandem payne/dakin reaction. |
| In vitro | HKPerox-2 is a O-methyl rhodol derivative and reaches a plateau within 30 min upon treatment of 100 μM H 2 O 2 , and the rate constants k obs is determined to be 2.0x10 -2 s for HKPerox2[1].HKPerox-2 (0-20 μM; 24 hours) is nontoxic to cells at working concentrations in RAW264.7 macrophages[1].HKPerox-2 should be co-incubated with 100 μM of trichloroacetonitrile (CCl3CN, HY-Y0789) for H 2 O 2 detection and molecular imaging. DMSO should be avoided during H 2 O 2 detection, as DMSO could scavenge activated H 2 O 2 in the presence of CCl3CN . DMF is the recommended solvent to prepare probe stock solution. |
| molecular weight | N/A |
| CAS | 2245230-79-7 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Sen Ye, et al. Tandem Payne/Dakin Reaction: A New Strategy for Hydrogen Peroxide Detection and Molecular Imaging. Angew Chem Int Ed Engl. 2018 Aug 6;57(32):10173-10177. |