| Description | HKPerox-1 is a yellow-emitting fluorescent probe with excellent selectivity and sensitivity toward H 2 O 2 . HKPerox-1 can be used for molecular imaging of endogenous H 2 O 2 in living cells. |
| In vitro | HKPerox-1 (1-20 μM; 24 h) is nontoxic to RAW264.7 macrophages[1]. HKPerox-1 (10 μM; 30 min) robustly visualizes endogenous H 2 O 2 production in macrophages in a selective manner[1]. HKPerox-1 should be co-incubated with 100 μM of trichloroacetonitrile (CCl3CN, HY-Y0789) for H 2 O 2 detection and molecular imaging. DMSO should be avoided during H 2 O 2 detection, as DMSO could scavenge activated H 2 O 2 in the presence of CCl3CN . DMF is the recommended solvent to prepare probe stock solution. |
| molecular weight | 714.683 |
| Molecular formula | C40H30N2O11 |
| CAS | 2240163-05-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Ye S, et, al. Tandem Payne/Dakin Reaction: A New Strategy for Hydrogen Peroxide Detection and Molecular Imaging. Angew Chem Int Ed Engl. 2018 Aug 6;57(32):10173-10177. |