| Description | HKOCl-4 (BXY2142) is a rhodol-based yellow fluorescent probe that demonstrates exceptional sensitivity and selectivity in detecting hypochlorous acid. Its absorption wavelength is significantly longer than that of fluorescein-based probes, and it exhibits better pH stability. Notably, HKOCl-4 exhibits excitation at 530 nm and emission at 557 nm. |
| In vitro | HKOCl-4 (10 μM) shows an increasing florescence intensity with increasing concentration of hypochlorous acid[1].HKOCl-4 exhibits a ultra-fast response towards 1 equiv of HOCl (reached a plateau within 2 min) , and it shows a >20-fold increase in fluorescence intensity over other analytes (1 equiv of ?OH and ONOO? as well as 10 equiv of O2, NO, 1O2, ROO?, TBHP and H2O2)[2].HKOCl-4 is first tested and it was proven to be non-toxic in living cells even at a concentration up to 50 μM in RAW264.7 macrophages[2]. |
| Synonyms | HKOCl-4, BXY2142 |
| molecular weight | 688.6 |
| Molecular formula | C38H35Cl2NO7 |
| CAS | 2031170-85-9 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Dan Yang, et al. Diarylether-based fluorogenic probes for detection of hypochlorous acid or hydroxyl radical. Patent US20160312033. 2. Xiaoyu Bai, et al. HKOCl-4: a rhodol-based yellow fluorescent probe for the detection of hypochlorous acid in living cells and tissues. |