| Description | HEPES Sodium salt is a nonvolatile zwitterionic chemical buffering agent. HEPES Sodium salt is broadly applied in cell culture. HEPES Sodium salt is effective at pH 6.8 to 8.2. HEPES Sodium salt also induces of lysosome biogenesis. |
| In vitro | HEPES Sodium salt maintains superhydrophilicity of titanium for at least 3 months, leading to a continuous retention of bioactivity and osteoconductivity[1]. In cultured RAW264.7 cells, HEPES Sodium salt drives lysosome biogenesis, affects MiT/TFE cytoplasmic-nuclear distribution, disrupts global cellular transcriptional profiles, leading to the activation of a MiT/TFE-dependent lysosomal-autophagic gene network[3]. |
| molecular weight | 260.29 |
| Molecular formula | C8H17N2NaO4S |
| CAS | 75277-39-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: Soluble DMSO: 50 mg/mL (192.09 mM), Sonication is recommended. |
| References | 1. Suzuki T, et al. Nonvolatile buffer coating of titanium to prevent its biological aging and for drug delivery. Biomaterials. 2010;31(18):4818-4828. 2. Sledź P, et al. An experimental charge density of HEPES. Acta Crystallogr B. 2010;66(Pt 4):482-492. 3. Tol MJ, van der Lienden MJC, Gabriel TL, Hagen JJ, Scheij S, Veenendaal T, Klumperman J, Donker-Koopman WE, Verhoeven AJ, Overkleeft H, Aerts JM, Argmann CA, van Eijk M. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution. Autophagy. 2018;14(3):437-449. doi: 10.1080/15548627.2017.1419118. Epub 2018 Feb 17. PMID: 29455584; PMCID: PMC5915011. |