| Description | HBC514, a nonfluorescent HBC-analog, emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. |
| In vitro | 胡椒能够使HBC的荧光增强超过3000倍，并在活细胞中表达时展示出明亮的荧光。在活的HeLa细胞中（表达sgRNA-Pep1和dCas9），通过基于胡椒的CRISPR成像，HBC514（2-10 μM）能够通过简单的洗涤和染色灵活变化。 |
| molecular weight | 330.43 |
| Molecular formula | C21H22N4 |
| CAS | 2530161-67-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 12.5 mg/mL (37.83 mM), Sonication is recommended. |
| References | 1. Chen X, et al. Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.Nat Biotechnol. 2019 Nov;37(11):1287-1293. |