| Description | Harringtonine is a naturally occurring alkaloid that inhibits DNA synthesis and protein synthesis and strongly kills S-phase cells. Harringtonine has antitumor and antiviral activities. |
| In vitro | 方法: HepG2/C3A 和 HuH-7.5 细胞用 Harringtonine (0.001-80 µM) 处理 24 h，通过 resazurin 染色检测细胞活力。结果: Harringtonine 在 0.12 µM-80 µM 的浓度范围内降低了细胞存活率，而在 0.001 µM 时没有观察到任何影响。在 Harringtonine 处理中观察到的活细胞百分比在 54%-63.7% 之间。[1]方法: Vero 细胞感染 ZIKV (1000 FFU)，并与 Harringtonine (156-625 nM) 一起孵育 48 h，分别通过 RT-qPCR、western blot 和 FFA 测定细胞内病毒 RNA 水平、蛋白质表达水平和上清液中的病毒子代。结果: Harringtonine 的剂量依赖性抗 ZIKV 活性降低了病毒 RNA/蛋白质的产生和后代产量，表明病毒繁殖受到抑制。IFA 测定结果还表明，Harringtonine 治疗以剂量依赖的方式抑制 ZIKV 感染。[2] |
| In vivo | 方法: 为研究体内活性，将 Harringtonine (0.5-4 mg/mouse) 眼眶后注射给 C57BL/6 小鼠。结果: 在 5 min 内，Harringtonine 完全耗尽了除睾丸和大脑外的所有器官中的多聚体，由于血脑和血睾丸屏障，需要长达 30 min 才能显著减少多聚体的数量。[3] |
| Synonyms | 三尖杉酯碱, 三尖杉脂碱 |
| molecular weight | 531.59 |
| Molecular formula | C28H37NO9 |
| CAS | 26833-85-2 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 55 mg/mL (103.46 mM) |
| References | 1. Franco DP, et al. Apoptotic and cell cycle response to homoharringtonine and harringtonine in wild and mutant p53 hepatocarcinoma cells. Hum Exp Toxicol. 2020 Oct;39(10):1405-1416. 2. Lai ZZ, et al. Harringtonine Inhibits Zika Virus Infection through Multiple Mechanisms. Molecules. 2020 Sep 7;25(18):4082. 3. Gerashchenko MV, et al. Translation elongation rate varies among organs and decreases with age. Nucleic Acids Res. 2021 Jan 25;49(2):e9. |