| Description | Gol-NTR, a highly selective and sensitive Golgi-targetable probe, is activated by Nitroreductase (NTR) to visualize acute lung injury (ALI) and its repair. With a low detection limit of 54.8 ng/mL, Gol-NTR is well-suited for research focused on monitoring and assessing the response of sepsis-induced ALI [1]. |
| In vitro | Gol-NTR 对 NTR 的荧光标记进行了明确化，通过使用DMSO溶剂制备了浓度为1.0 mM的Gol-NTR母液。[1]（1） |
| In vivo | 体内成像[1]过程描述：首先对C57BL/6雄性小鼠(6-8周龄，体重20-22g)预先注射300μL DMOG (25 mg/mL)，经过24小时，再腹腔注射300μL LPS (10 mg/kg)并持续6小时。随后依照操作步骤(2)对小鼠实施颈椎脱臼处死，并收集肺组织。接着依携步骤(3)使用PBS进行洗涤后，用浓度为50μM的Gol-NTR在PBS溶液中孵化1小时。最后根据步骤(4)再次用PBS进行清洗，并采用小型动物成像系统，使用420nm激发波长与510nm发射波长进行荧光成像。 |
| molecular weight | N/A |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |