| Description | FITC-Dextran (MW 10000) is a marker (10 kDa) formed by coupling FITC to dextran. FITC-Dextran is a labeled polysaccharide composed of branched glucose molecules of various lengths, enabling the determination of solute, ionic, and protein permeability of the blood-brain barrier depending on the size of the dextran used. |
| In vitro | 方法: FITC-dextran 检测 RBL-2H3 肥大细胞系：1、每 1 mL 培养基混合 1 mg FITC-dextran 粉末。使用孔径为 0.22 µm 的醋酸纤维素注射器过滤装置，过滤溶解的 FITC-dextran。2、将 10 µL 细胞悬浮液加入预先填充有新鲜 FITC-dextran 补充培养基的腔室盖玻片中。3、在 37℃、5% CO2 的潮湿环境中培养 RBL 细胞过夜。细胞应保持在亚融合水平，以确保细胞分离，并且在显微镜下很容易单独识别每个细胞。4、通过从腔室中吸出介质并重新填充 300 µL 预热至 37℃ 的 Tyrode 缓冲液，清洗腔室盖玻片 3 次。最后，向腔室中补充 300 µL 预热至 37℃ 的 Tyrode 缓冲液。5、选择要跟踪的感兴趣区域，请打开荧光光源并选择合适的荧光滤光片，选择绿色荧光团的滤光片以查看 FITC-dextran。如果使用基于激光的显微镜，请打开 488 nm激光，发射应集中在 500-550 nm 左右。[1] |
| In vivo | 方法: 使用 FITC-dextran 测定肠通透性：1、将 FITC-dextran 以 80 mg/mL 的浓度溶解在灭菌的 PBS 中。2、在注射 FITC-dextran 之前，小鼠禁食 4 h。随后口服 100 µL FITC-dextran。3、4 h 后，收集小鼠血液，以 4000 rpm 离心 15 min。将血浆样本收集到透明的 EP 管中，避光置于 4℃。4、标准 FITC-dextran 样品用 PBS 稀释为 0-100 µg/mL，并转移到黑色 96 孔板上。5、使用微孔板读数器在 485 nm 的激发波长和 528 nm 的发射波长下测量荧光。[2] |
| Synonyms | FITC-Dextran, 荧光素异硫氰酸酯-葡聚糖(MW 10000), 荧光素异硫氰酸酯-葡聚糖 |
| molecular weight | 10000 |
| CAS | 60842-46-8 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: 50 mg/mL, Sonication is recommended. |
| References | 1. Klein O, et al. Imaging FITC-dextran as a Reporter for Regulated Exocytosis. J Vis Exp. 2018 Jun 20;(136):57936. 2. Jeong YJ, et al. A derivative of 3-(1,3-diarylallylidene)oxindoles inhibits dextran sulfate sodium-induced colitis in mice. Pharmacol Rep. 2024 Jun 25. 3. Eriksson I, et al. Analysis of Lysosomal pH by Flow Cytometry Using FITC-Dextran Loaded Cells. Methods Mol Biol. 2017;1594:179-189. |
| Citations | 1. Wang Y, Pang J, Wang Q, et al. Delivering Antisense Oligonucleotides across the Blood‐Brain Barrier by Tumor Cell‐Derived Small Apoptotic Bodies. Advanced Science. 2021, 8(13): 2004929. 2. Li Y, Liu C, Chen Z, et al.Netrin‐1 protects blood–brain barrier (BBB) integrity after cerebral ischemia‐reperfusion by activating the Kruppel‐like factor 2 (KLF2)/occludin pathway.Journal of Biochemical and Molecular Toxicology.2024, 38(1): e23623. |