Doxycycline Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	Doxycycline (Doxiciclina) belongs to the tetracycline class of antibiotics and is a broad-spectrum metalloproteinase (MMP) inhibitor with oral activity. Doxycycline has antibacterial activity and antitumor activity.
In vitro	方法：22 种肿瘤细胞用 Doxycycline 处理 48 h，使用 MTT 方法检测细胞活力。结果：肺癌细胞对 Doxycycline 比其他大多数细胞系更敏感。NCI-H446 和 A549 细胞对 Doxycycline 敏感，IC50分别为 1.70 μM 和 1.06 μM。[1]方法：人胰腺癌细胞 PANC-1 用 Doxycycline (10-40 μg/mL) 处理 8-24 h，使用 Flow Cytometry 方法检测细胞周期情况。结果：Doxycycline 使细胞停滞在细胞周期的 G1-S 期。[2]
In vivo	方法：为检测体内抗肿瘤活性，将 Doxycycline (50 mg/kg) 腹腔注射给携带人乳腺癌肿瘤 4T1 的 BALB/c 小鼠，每天一次，持续十五天。结果：Doxycycline 延迟肿瘤生长并降低肿瘤相关巨噬细胞和血管的数量。[3]方法：为检测体内抗菌活性，将 Doxycycline (40 mg/kg) 腹腔注射给暴露于 B. pseudomallei 1026b 的 BALB/c 小鼠，每天两次，持续两周。结果：用 Doxycycline 治疗的小鼠 100% 存活，并且从大多数存活小鼠的肺或脾脏中没有扩增出 B. pseudomallei 的 DNA。[4]
Synonyms	Vibramycin, 多西环素, Doxiciclina, Doxytetracycline, Doxycyclinum, 强力霉素
molecular weight	444.43
Molecular formula	C22H24N2O8
CAS	564-25-0
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year
Solubility	DMSO: 50 mg/mL (112.5 mM), Sonication is recommended.
References	1. Qin Y, et al. Doxycycline reverses epithelial-to-mesenchymal transition and suppresses the proliferation and metastasis of lung cancer cells. Oncotarget. 2015 Dec 1;6(38):40667-79. 2. Son K, et al. Doxycycline induces apoptosis in PANC-1 pancreatic cancer cells. Anticancer Res. 2009 Oct;29(10):3995-4003. 3. Tang X, et al. Doxycycline attenuates breast cancer related inflammation by decreasing plasma lysophosphatidate concentrations and inhibiting NF-κB activation. Mol Cancer. 2017 Feb 8;16(1):36. 4. Gelhaus HC, et al. Efficacy of post exposure administration of doxycycline in a murine model of inhalational melioidosis. Sci Rep. 2013;3:1146.
Citations	1. Tan Y, Chen X, Xu Z, et al.The mechanics of tumor cells dictate malignancy via cytoskeleton-mediated APC/Wnt/β-catenin signaling.Research.2023 2. Li Y M, Mei Y C, Liu A H, et al.Gcn5-and Bre1-mediated Set2 degradation promotes chronological aging of Saccharomyces cerevisiae.Cell Reports.2023, 42(10). 3. Wang W, Wang M, Du T, et al.SHMT2 Promotes Gastric Cancer Development through Regulation of HIF1α/VEGF/STAT3 Signaling.International Journal of Molecular Sciences.2023, 24(8): 7150. 4. Lu J, Lu Z, Liu L, et al. Identification of crocin as a new hIAPP amyloid inhibitor via a simple yet highly biospecific screening system. Chemistry & Biodiversity. 2021 5. Xin Y, Chen X, Tang X, et al. Mechanics and actomyosin-dependent survival and chemoresistance of suspended tumor cells in shear flow. Biophysical Journal. 2019 May 21;116(10):1803-1814 6. Li Y, Qiao K, Zhang X, et al. Targeting myeloid-derived suppressor cells to attenuate vasculogenic mimicry and synergistically enhance the anti-tumor effect of PD-1 inhibitor. Iscience. 2021, 24(12): 103392. 7. Chen X, Fan Y, Sun J, et al. Nanoparticle-mediated specific elimination of soft cancer stem cells by targeting low cell stiffness. Acta Biomaterialia. 2021 8. Han L, Jiang Y, Shi M, et al.LIPH contributes to glycolytic phenotype in pancreatic ductal adenocarcinoma by activating LPA/LPAR axis and maintaining ALDOA stability.Journal of Translational Medicine.2023, 21(1): 1-21. 9. Hu Z, Lu Y, Cao J, et al.N-acetyltransferase NAT10 controls cell fates via connecting mRNA cytidine acetylation to chromatin signaling.Science Advances.2024, 10(2): eadh9871. 10. Wang J, Zhang B, Chen X, et al.Cell mechanics regulate the migration and invasion of hepatocellular carcinoma cells via JNK signaling.Acta Biomaterialia.2024

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Doxycycline

CAS No.: 564-25-0