| Description | Diphyllin could be characterized as a new V-ATPase inhibitor in treating gastric cancer and inhibiting the phosphorylation of LRP6 in Wnt/β-catenin signaling. |
| In vitro | The in vitro inhibitory effects of these compounds were evaluated on the production of nitric oxide (NO) and cytokines (tumor necrosis factor (TNF)-alpha and interleukin (IL)-12), from LPS/IFN-gamma activated murine peritoneal macrophages. The results indicated that the 50% inhibition concentration (IC(50)) values of NO production from activated peritoneal macrophages by compounds 1-4 were 100, 25, 12.5 and 50 microM, respectively. In parallel, these dilutions were approximately inhibited in a similar manner to that observed for cytokines (TNF-alpha, and IL-12) production. On the other hand, at 100 microM concentration compounds 3 and 4 showed 50% inhibition of NO production from peritoneal macrophages that had been pre-activated with LPS/IFN-gamma for 24h, whereas compounds 1 and 2 inhibited only about 20 and 10%, respectively. |
| Target activity | HIV-1:0.38 μM (IC50), H+-ATPase (vacuolar type):17 nM (IC50) |
| Synonyms | 山荷叶素, 二叶草素 |
| molecular weight | 380.35 |
| Molecular formula | C21H16O7 |
| CAS | 22055-22-7 |
| Storage | keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMSO: 3.8 mg/mL (10 mM) |
| References | 1. Anti-inflammatory activities of constituents isolated from Phyllanthus polyphyllus.J Ethnopharmacol. 2006 Jan 16;103(2):181-6. |