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DiI triflate [1,1‘-Dioctadecyl-3,3,3’,3‘-tetramethylindocarbocyanine triflate]

CAS No.:

DiI triflate is one of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluore
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Description DiI triflate is one of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water.
In vitro Stain adherent cells: Grow adherent cells on sterile glass coverslips. Remove coverslips from growth medium and gently drain off excess medium. Place coverslip in a humidity chamber. Pipet 100 μL of the dye working solution onto the corner of a coverslip and gently agitate until all cells are covered. Incubate the coverslip at 37℃ for 2-20 minutes. Drain off the dye working solution and wash the coverslips two to three times with pre-warmed growth medium. Microscopy Detection: DiI, 549/565 nm, XF32-Omega, 31002-Chroma; DiR, 748/780 nm, XF112-Omega, 41009-Chroma
molecular weight N/A
Storage keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year