| Description | Digitonin is a natural product of the glycoside family and is a detergent. Digitonin has antitumor activity, binds to cholesterol molecules to increase the permeability of cell membranes, and is active in the cleavage of cell membranes in a wide range of cells. |
| In vitro | 方法：大鼠肾上腺嗜铬细胞瘤细胞 PC-12 用 Digitonin (10 μg/mL) 处理 10 min，增加细胞通透性。结果：在用 Digitonin 透化后，可以通过微摩尔浓度的游离 Ca2+ 刺激 PC12 细胞释放去甲肾上腺素。[1]方法：人胶质母细胞瘤细胞 U87 用 Digitonin (1-5 μg/mL) 处理 20 min，细胞孵育过夜后使用 MTT 方法检测细胞活力。结果：1 μg/mL Digitonin 处理导致细胞活力轻微增加。3 μg/mL Digitonin 可减少促生长作用，细胞活力与对照相似。5 μg/mL Digitonin 会抑制细胞活力，表明对细胞有显著毒性。[2]方法：永生化的人类 T 淋巴细胞 Jurkat 用 Digitonin (1-4 μg/mL) 处理 20 min，使用 Resazurin assay 检测细胞活力。结果：Digitonin 处理导致代谢活性显著降低，并且这种抑制作用具有浓度依赖性。[2] |
| In vivo | 方法：为确定 Digitonin 是否能在体内提高 CBDCA 的疗效，将 Digitonin (0.2 mL 400 μM) 肝动脉输注给携带肝癌肿瘤的 LH 大鼠，10 min 后注射 CBDCA。结果：Digitonin 预处理增加了 CBDCA 的肿瘤摄取，并增强了 CBDCA 的细胞毒性作用。[3] |
| molecular weight | 1229.31 |
| Molecular formula | C56H92O29 |
| CAS | 11024-24-1 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 27.5 mg/mL (22.37 mM) |
| References | 1. Schäfer T, et al. Digitonin-permeabilized cells are exocytosis competent. J Neurochem. 1987 Dec;49(6):1697-707. 2. Wang S, et al. Digitonin-facilitated delivery of imaging probes enables single-cell analysis of AKT signalling activities in suspension cells. Analyst. 2021 Sep 7;146(17):5307-5315. 3. Lindnér PG, et al. Digitonin enhances the efficacy of carboplatin in liver tumour after intra-arterial administration. Cancer Chemother Pharmacol. 1997;40(5):444-8. |
| Citations | 1. Chen Y, Wang X, Shi H, et al. Montelukast Inhibits HCoV-OC43 Infection as a Viral Inactivator. Viruses. 2022, 14(5): 861 2. He Z, Zhang Z, Xu P, et al.Laminarin Reduces Cholesterol Uptake and NPC1L1 Protein Expression in High-Fat Diet (HFD)-Fed Mice.Marine Drugs.2023, 21(12): 624. |