| Description | Dibenzylfluorescein (DBF), a fluorogenic probe (Fluorescent dye), serves as a substrate for various cytochrome P450 (CYP) isoforms, such as CYP3A4, CYP2C8, CYP2C9, CYP2C19, and aromatase (CYP19). It is commonly utilized at its K_m value range of 0.87-1.9 µM (Ex=485nm, Em=535nm) to monitor alterations in CYP enzymatic activity attributable to pharmaceutical interventions or pathophysiological conditions [1] [2] [3] [4]. |
| In vitro | P450催化的Dibenzylfluorescein代谢及氢氧化钠影响的实验方法[3]如下：Dibenzylfluorescein在P450的作用下脱烷基形成荧光素苄基酯，氢氧化钠的加入能促进其水解为荧光素；而2M氢氧化钠可直接将Dibenzylfluorescein分解为荧光素苄基醚。实验条件包括：1) CYP2C19酶活性及抑制反应，将0.1M Tris-HCl (pH 7.4)、10 μM Dibenzylfluorescein、15 pmol CYP2C19和50 μL NADPH再生系统混合，形成150 μL反应体系。NADPH再生系统包含1.13 mM NADP、12.5 mM异柠檬酸、56.33 mM KCl、187.5 mM Tris-HCl (pH 7.4)、12.5 mM MgCl2、0.0125 mM MnCl2和0.075 U/ml异柠檬酸脱氢酶。2)样品在37°C下孵育30至60分钟，然后迅速冷却至4°C以终止反应，并经离心后取上清液进行LC-MS分析。3)对照分析在无氢氧化钠和加入2M氢氧化钠的条件下，使用纯Dibenzylfluorescein、荧光素苄酯、荧光素苄醚及荧光素（浓度均为10 μM）作为标准品。 |
| Synonyms | DBF |
| molecular weight | 512.55 |
| Molecular formula | C34H24O5 |
| CAS | 97744-44-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |