| Description | DFHBI is a small molecule, resembles the chromophore of green fluorescent protein (GFP). Spinach and DFHBI are essentially nonfluorescent when unbound. The Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells. |
| In vitro | 菠菜和菠菜2与DFHBI结合时，具有447 nm的荧光激发峰和501 nm的荧光发射峰[1]，它们是可以用于遗传编码荧光RNA的RNA适体。菠菜是一个98-nt长的RNA适体，能够结合DFHBI并激活其荧光。当未结合时，菠菜和DFHBI基本上不发荧光，而菠菜-DFHBI复合体在体外和活细胞中则表现出明亮的荧光。菠菜2能够结合并激活DFHBI的荧光，使得可以在活细胞中成像观察菠菜2标记的RNA的动态定位。菠菜2的光谱特性受DFHBI限制，后者产生的荧光为蓝绿色，并不适用于荧光显微镜中常用的滤光片。应避免DFHBI暴露于光照。所有DFHBI的原液应保存在黑暗的试管中或用箔纸包裹。含有DFHBI的培养皿应使用箔纸包裹以避免光照[2]。 |
| molecular weight | 252.22 |
| Molecular formula | C12H10F2N2O2 |
| CAS | 1241390-29-3 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 83.33 mg/mL (330.39 mM) |
| References | 1. Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. 2. Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9(1):146-55. |