| Description | DFHBI-1T is an RNA aptamer-activated fluorescent probe that transmits through cell membranes.DFHBI-1T can be used for dynamic localization imaging of RNA in living cells. |
| In vitro | DFHBI-1T (20 μM; 10 分钟) 在 COS7 细胞中增强了 (CGG)60-Spinach2 的荧光表达，与 DFHBI (20 μM) 相比[2]。具体的荧光特性为：Broccoli-DFHBI-1T 的激发/发射波长为 472 nm/507 nm，而 Spinach2-DFHBI-1T 的激发/发射波长为 482 nm/505 nm[1]。 |
| Synonyms | DFHBI1T, DF-HBI-1T, DF HBI 1T, DFHBI 1T |
| molecular weight | 320.21 |
| Molecular formula | C13H9F5N2O2 |
| CAS | 1539318-36-9 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 80 mg/mL (249.84 mM), Sonication is recommended. |
| References | 1. Grigory S Filonov, et al. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308. 2. Wenjiao Song, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. |