D-α-Hydroxyglutaric acid disodium Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	D-α-Hydroxyglutaric acid disodium (Disodium (R)-2-Hydroxyglutarate) is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases with Ki of 0.628 mM.
In vitro	In U-87 mg cells, (R)-2-Hydroxyglutarate acts as weak antagonists of α-KG to inhibit α-KG-dependent histone demethylases and increases dimethylation on both H3K9 and H3K79. [1] Besides, (R)-2-Hydroxyglutarate inhibits ATP synthase and mTOR signaling, and thus causes growth arrest and tumor cell killing. [2]
Cell experiments	Cells are seeded in 12-well plates and, after overnight incubation, treated with the indicated concentrations of each compound. After harvesting, cells are stained with acridine orange (AO) and 4′,6-diamidino-2-phenylindole (DAPI). Cell number and viability are measured based on AO and DAPI fluorescence as measured by NC3000 following the manufacturer's instructions.(Only for Reference)
Target activity	α-ketoglutarate-dependent dioxygenase:0.628 mM(Ki)
Synonyms	D-alpha-Hydroxyglutaric acid disodium salt, (R)-2-羟基戊二酸二钠盐, Disodium (R)-2-Hydroxyglutarate
molecular weight	192.08
Molecular formula	C5H6Na2O5
CAS	103404-90-6
Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year
Solubility	DMSO: < 1mg/ml (insoluble）
References	1. Xu W, et al. Cancer Cell. 2011, 19(1), 17-30. 2. Kim SY, et al. Cardiovasc Res. 2011, 92(2), 307-316.

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