| Description | Cyclic-di-GMP disodium (5GP-5GP disodium) is a bacterial second messenger and a STING agonist involved in a variety of prokaryotic processes including biofilm formation, motility, and cell cycle progression.Cyclic-di-GMP disodium displays antiproliferative activity against cancer cells, inducing elevated CD4 receptor expression and cell cycle arrest. Cyclic-di-GMP disodium can be used in cancer research cancer. |
| In vitro | Cyclic-di-GMP disodium (0.5-50 μM; 5 days) demonstrates inhibition of human colon cancer cell proliferation.[1]Cyclic-di-GMP disodium (0.5-50 μM; 5 days) induces a targeted increase in CD4 expression within Jurkat cells and triggers S-phase cell cycle arrest in the same cells.[2] |
| In vivo | Cyclic-di-GMP disodium (100 μg/per; i.v.; two sequential vaccinations 9 days apart) augments TriVax-induced immune responses against melanoma in mice and further amplifies the anti-tumor effects of TriVax.[3] |
| Synonyms | Cyclic diguanylate, 5GP-5GP disodium, 3',5'-Cyclic diguanylic acid, Cyclic di-GMP (sodium salt), cyclic diguanylate disodium, c-di-GMP, c-di-GMP disodium |
| molecular weight | 734.38 |
| Molecular formula | C20H22N10Na2O14P2 |
| CAS | 2222132-40-1 |
| Storage | store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 144.0 mg/mL (196.1 mM), Sonication is recommended. |
| References | 1. Karaolis DK, et al. 3',5'-Cyclic diguanylic acid (c-di-GMP) inhibits basal and growth factor-stimulated human colon cancer cell proliferation. Biochem Biophys Res Commun. 2005;329(1):40-45. 2. Steinberger O, et al. Elevated expression of the CD4 receptor and cell cycle arrest are induced in Jurkat cells by treatment with the novel cyclic dinucleotide 3',5'-cyclic diguanylic acid. FEBS Lett. 1999;444(1):125-129. 3. Wang Z, et al. STING activator c-di-GMP enhances the anti-tumor effects of peptide vaccines in melanoma-bearing mice. Cancer Immunol Immunother. 2015;64(8):1057-1066. 4. Jenal U, et al. Cyclic di-GMP: second messenger extraordinaire. Nat Rev Microbiol. 2017;15(5):271-284. |