| Description | CY5-SE Ditriethylamine salt is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. Excitation (nm):649, Emission (nm): 670. |
| In vitro | SELP类似物与Cy5-SE的偶联反应。将1 mL的100 μg/mL SELP溶液与10 μL的1.2 mg/mL Cy5 mono NHS-酯混合，后者溶于10% 的DMSO中，此混合液在100 mM的碳酸钠缓冲液（pH 8.3）中冰浴孵育2小时。反应终止时，向反应液中加入50 μL的1 M Tris-HCl（pH 8.0）。反应混合物装载到1.5 mL的Sephadex G-25柱上，通过离心（3分钟，1050g[1]）收集Cy5标记的SELPs。 |
| Synonyms | CY5-SE Ditriethylamine salt(146368-14-1 Free base) |
| molecular weight | 956.26 |
| Molecular formula | C49H73N5O10S2 |
| Storage | keep away from moisture|Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| References | 1. Jeon HY, et al. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405. |