| Description | Coelenterazine (Coelenteramine) is a luminescent enzyme substrate, used for monitoring reporter genes in BRET, ELISA and HTS techniques. |
| In vitro | 在监测多种生物学过程时，coelenterazine (CTZ) 可以被海葵 Renilla reniformis (Rluc) 和海洋桡足类 Gaussia princeps (Gluc) 等luciferases 催化，产生蓝色光线发射。在表达Gli36-Gluc或Gli36-Rluc的Gli36细胞中，coelenterazine展现出生物发光信号。[1] 在RIN-5F、4T1、HCT 116、HEK 293、293FT、HeLa 和 INS-1细胞中，coelenterazine可用于检测超氧阴离子浓度。[2] |
| In vivo | 在4T1植入的小鼠中，Coelenterazine检测与癌症相关的超氧阴离子，并用于评估超氧阴离子在癌细胞生物学及其病理作用中的角色。[2] |
| Cell experiments | Cells are plated at a density of 5×10^4?cells per well into 24-well plates and grown to 80-100% confluency. Just before imaging, media are changed to a colorless solution containing (in mM): 2.7 KCl, 139 NaCl, 8.1 Na2HPO4, 0.7 H2O, 1.5 KH2PO4, 1.8 CaCl2, 1 MgCl2?and 5.5 d-glucose. Cells are preincubated for 15 min in the absence or presence of Pgp modulator, after which Coelenterazine (final concentration of 470 nM) is added directly to the cells. They are for reference only. |
| Animal experiments | Mice are anesthetized with metofane or isoflurane before tail vein injection of Coelenterazine (4 μg/g) formulated from an ethanol stock diluted in sodium phosphate buffer (50 mM). Bioluminescence imaging is performed on the in vivo imaging system at 2, 6, 8, and 11 min after injection. Anesthesia is maintained during imaging by nose cone delivery of 2.5% isoflurane. After imaging, animals are killed by cervical dislocation; tumors are then harvested and weighed. They are for reference only. |
| Synonyms | Oplophorus luciferin, Coelenteramine, 腔肠素 |
| molecular weight | 423.46 |
| Molecular formula | C26H21N3O3 |
| CAS | 55779-48-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | Ethanol: 1 mg/mL (2.36 mM), DMSO inactivates the activity of Coelenterazine. H2O: < 1 mg/mL (insoluble or slightly soluble) |
| References | 1. Morse D, et al. Mol Ther. 2012, 20(4), 692-693. 2. Bronsart LL, et al. Mol Imaging Biol. 2015 |
| Citations | 1. Hong Y, Guo H, Wei M, et al. Cell-based reporter assays for measurements of antibody-mediated cellular cytotoxicity and phagocytosis against SARS-CoV-2 spike protein. Journal of Virological Methods. 2022: 114564 |