| Description | Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form citrulline, which plays a large role in regulating gene expression. Abnormally high PAD activity has been observed in a host of human diseases. Citrulline-specific probe-rhodamine is a highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline. This chemical probe (comprised of a single isomer) is capable of reacting with any citrulline-containing protein and can be analyzed with fluorescent imaging (excitation 532 nm; emission 580 nm). When added at 100 μM for 30 minutes at acidic pH, this probe has a reported limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4. |
| molecular weight | 731.75 |
| Molecular formula | C39H37N7O8 |
| CAS | 2309313-01-5 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | DMF: 1 mg/mL Acetonitrile:H2O (2:1): 1 mg/mL |
Citrulline-specific Probe-Rhodamine
CAS No.: 2309313-01-5
Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form
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