| Description | Cephradine (Anspor) is a beta-lactam, first-generation cephalosporin antibiotic with bactericidal activity. |
| In vivo | Cephradine 的急性、亚急性和慢性口服毒性以及急性和亚急性肠外毒性较低。每天给猴子静脉注射Cephradine 2 周后，这些动物没有出现肾毒性症状。Cephradine 不会对小鼠或大鼠的后代产生任何致畸变化。Cephradine 这种单一的头孢菌素抗生素既可用于口服制剂，也可用于肠外制剂，这是一种明显的治疗优势[2]。 |
| Cell experiments | Cells Are seeded in six-well culture plates (9.6 cm2, growth area) at a density of 105 cells/cm2. At 2 days postseeding, the medium is removed and cells are washed twice with pH 6.0 uptake buffer. Independent studies are performed for each drug solution at 1 mg/mL made in pH 6.0 uptake buffer. At each time point (10, 20, 30, 45, 60, 90 min), drug solution is removed and cells are washed three times with ice-cold pH 6.0 uptake buffer. One milliliter of Milli-Q water is added to each well and incubated for 30 min at 25 °C. Cells are harvested and sonicated for 1-2 min. ZnSO4 solution (8%, 200 mL) is added to the cell lysate, vortexed rigorously, and centrifuged for 5 min at 3000 rpm. After filtration of the supernatant through a membrane filter (0.45 mm), samples are analyzed by HPLC. (Only for Reference) |
| Synonyms | Anspor, Cephradin, Velosef, SQ-11436, Sefril, 头孢拉定 |
| molecular weight | 349.4 |
| Molecular formula | C16H19N3O4S |
| CAS | 38821-53-3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year |
| Solubility | H2O: 8.33 mg/mL (23.84 mM), Sonication is recommended. DMSO: 3.6 mg/mL (10.30 mM) |
| References | 1. Han HK, et al. J Pharm Sci. 1999, 88(3):347-50. 2. Hassert GL, et al. Antimicrob Agents Chemother. 1973 Jun;3(6):682-5. |