| Description | Calcipotriol monohydrate is an analog of Vitamin D3 with a high affinity for the vitamin D receptor. Calcipotriol monohydrate can be used for studies about psoriasis. |
| In vitro | Calcipotriol monohydrate（100, 10, 和 1 ng/mL）显著提升了NK细胞表面NKp30的表达量，在4小时的孵育后[1]。当NHEK细胞未被IL-17A或IL-22刺激时，Calcipotriol monohydrate轻微增强（0.2 nM）IL-8 mRNA表达，或无影响（2-20 nM）。加入IL-17A和IL-22后，IL-8的mRNA表达显著提升，证实了我们之前的研究。这种IL-8 mRNA表达的增强，通过Calcipotriol monohydrate（2, 20 和 40 nM）以剂量依赖方式被抑制[2]。 |
| In vivo | 在经过Diclofenac加Calcipotriol monohydrate（p=0.018）和Diclofenac加DFMO加Calcipotriol monohydrate（p=0.002）处理的组别中，与安慰剂（线性回归模型）[3]相比，体重增加显著较小。 |
| Cell experiments | Normal human epidermal keratinocytes are grown in serum-free keratinocyte growth medium Epilife and used at the third passage in all experiments. The growth supplement is omitted 48 h before experiments. As a control, IL-17A and IL-22 are either added or not added to the cells. Cultured NHEK cells are stimulated with IL-17A (200 ng/mL) and/or IL-22 (200 ng/mL) followed by co-incubation in the presence or absence of Calcipotriol at 0.2-40 nM to test its modulatory effect. Cells are harvested 3 days later and subjected to real-time qPCR. Culture supernatants are also collected and frozen at -80°C until used for ELISA [1]. |
| Animal experiments | The 160 female SKH-1 hairless mice (6-7 weeks of age) are used. After UV treatment, mice without tumors are randomly divided into five groups, four chemoprevention groups (Diclofenac plus DFMO; Diclofenac plus Calcipotriol; DFMO plus calcitriol; and Diclofenac plus DFMO plus Calcipotriol) and one placebo group (skin lotion). The mice are treated with test mixtures once a day, five days a week, for a total of 17 weeks. The test mixtures are applied topically on the dorsal surface of the mice. Ten microliters are applied by a pipette after which the mixture is rubbed onto the skin. This corresponded to the following doses of each active substance in the treatments: 100 μg/week for Diclofenac (30 mg/g undiluted), 0.166 μg/week for Calcitriol (50 μg/g undiluted), and 463.3 μg/week for DFMO (139 mg/g undiluted) [3]. |
| Synonyms | 钙泊三醇一水合物, 卡泊三醇一水合物 |
| molecular weight | 430.62 |
| Molecular formula | C27H42O4 |
| CAS | 147657-22-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | Ethanol: 95 mg/mL (220.61 mM), Sonication is recommended. DMSO: 95 mg/mL (220.61 mM), Sonication is recommended. |
| References | 1. Al-Jaderi Z, et al. Effects of vitamin D3, calcipotriol and FTY720 on the expression of surface molecules and cytolytic activities of human natural killer cells and dendritic cells. Toxins (Basel). 2013 Oct 28;5(11):1932-47. 2. Sakabe JI, et al. Calcipotriol Increases hCAP18 mRNA Expression but Inhibits Extracellular LL37 Peptide Production in IL-17/IL-22-stimulated Normal Human Epidermal Keratinocytes. Acta Derm Venereol. 2014 Sep;94(5):512-6. 3. Pommergaard HC, et al. Combination chemoprevention with diclofenac, calcipotriol and difluoromethylornithine inhibits development of non-melanoma skin cancer in mice. Anticancer Res. 2013 Aug;33(8):3033-9. |