| Description | Calcein Blue is a blue-fluorescent, short-term dye. |
| In vitro | Calcein blue AM在原始状态下的荧光较弱（激发/发射最大峰值为322/435 nm）。当细胞内酯酶切割AM酯后，该示踪剂变得相对极性并可被细胞保留数小时。此外，其荧光强度增加，并且荧光光谱向长波长方向移动，激发/发射最大峰值达到360/449 nm [1]。当在含Calcein blue–Fe2复合物的溶液中加入多巴胺时，由于Calcein blue与多巴胺间的配体交换反应，会形成多巴胺–Fe2复合物，从而恢复Calcein blue的荧光。在激发波长340 nm处，测量得到的荧光强度与加入的多巴胺浓度成正比，这使得能够对多巴胺进行定量分析 [2]。 |
| molecular weight | 321.28 |
| Molecular formula | C15H15NO7 |
| CAS | 54375-47-2 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 6 mg/mL (18.68 mM), Sonication and heating are recommended. |
| References | 1. Sankaranarayanan R, et al. A new fluorimetric method for the detection and quantification of siderophores using Calcein Blue, with potential as a bacterial detection tool. Appl Microbiol Biotechnol. 2015 Mar;99(5):2339-49. 2. Seto D, et al. A simple and selective fluorometric assay for dopamine using a calcein blue-Fe2+ complex fluorophore. Talanta. 2012 May 30;94:36-43. |