| Description | Calcein-AM is a cell-permeable fluorescent dye used for the determination of cell viability. |
| In vitro | The calcein-AM dye used to stain the living cells have a low spontaneous leakage rate of less than 15% in 4 hours at 37°C. Dilutions of targets stained by calcein-AM have a linear relationship with measured fluorescence values. NK cells, LAKs, and CTLs are readily detectable by this micro-test. Quantitation of killing and kinetic analysis is readily performed with the test system [1]. Calcein-AM is pH-independent, better retained, and more photostable. In addition, the high level of intracellular retention of calcein-AM and its low-level release after incorporation exclude possible cell-monolayer labeling and allow its use in a cell-cell interaction assay. Moreover, the bright fluorescence can easily be detected and measured by a microplate fluorescence reader [2]. Calcein-AM is a highly lipophilic vital dye that rapidly enters viable cells, is converted by intracellular esterases to calcein that produces an intense green (530-nm) signal, and is retained by cells with the intact plasma membrane. The calcein-AM assay has been used to assess cell viability, cytotoxicity, and tp quantitate apoptosis [3]. |
| Synonyms | Calcein acetoxymethyl ester, 钙黄绿素-AM |
| molecular weight | 994.86 |
| Molecular formula | C46H46N2O23 |
| CAS | 148504-34-1 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Wang XM, et al. A new microcellular cytotoxicity test based on calcein-AM release. Hum Immunol. 1993 Aug;37(4):264-70. 2. Braut-Boucher F, et al. A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calceinAM-labeled lymphocytes. J Immunol Methods. 1995 Jan 13;178(1):41-51. 3. Bratosin D, et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viabilityand aging. Cytometry A. 2005 Jul;66(1):78-84. |