| Description | C12FDG (5-Dodecanoylaminofluorescein di-beta-D-Galactopyranoside) is used as a lipophilic green fluorescent substrate for detecting beta-galactose activity, a green fluorescent substrate, is a senescence marker and is often used in conjunction with flow cytometry. |
| In vitro | 荧光老化相关β-半乳糖苷酶(SA-β-Gal)实验 [1]:1. 培养细胞，密度为105个/mL .2. 用200 μL PBS洗涤细胞1次，然后用100 μL固定液(2%甲醛/0.2%戊二醛蒸馏水)在室温下固定5分钟。3. 用200 μL PBS洗涤细胞2次，用100 μL 33 μM C12FDG (PBS,pH=6.0)染色10 min，再用200 μL Hoechst溶液(1 μg/mL Hoechst 33342)在PBS(pH 6.0)上染色10 min。4. 用20倍物镜和360 nm (Hoechst 33342)和480 nm (C12FDG)的激发滤光片对细胞成像，用460 nm和535 nm的发射滤光片对细胞进行监测。仅供参考，应根据您的实验具体需要进行修改。 |
| Synonyms | 5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside |
| molecular weight | 853.9 |
| Molecular formula | C44H55NO16 |
| CAS | 138777-25-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 30 mg/mL (35.13 mM), Sonication is recommended. |
| References | 1. Udono M, Kadooka K, Yamashita S, Katakura Y. Quantitative analysis of cellular senescence phenotypes using an imaging cytometer. Methods. 2012 Mar;56(3):383-8. |