| Description | Bromothymol Blue is a pH indicator. It is yellow when it is acidic and blue when it is alkaline. |
| In vitro | 将溴百里酚蓝溶液(0.1%,w/v)加入琼脂平板后,由于K. vulgare 07突变体在琼脂平板上释放2-KLG,使得菌落周围区域的pH值下降,导致K. vulgare 07突变体周围的区域由蓝色变为黄色[2]。 |
| molecular weight | 624.38 |
| Molecular formula | C27H28Br2O5S |
| CAS | 76-59-5 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | H2O: < 0.1 mg/mL (insoluble) DMSO: 90 mg/mL (144.14 mM), Sonication is recommended. |
| References | 1. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80. 2. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul - Sep;48(3):397-402. |