| Description | BODIPY-Cholesterol (TF-Chol) is a cholesterol analog with a fluorescent BODIPY motif and cellular permeability, and is a novel BODIPY cholesterol probe.BODIPY-Cholesterol can be used to study intracellular sterol uptake and interorganelle sterol flux. |
| In vitro | 针对BODIPY-Cholesterol测定：首先将类似物溶解于DMSO中，配制1-10 μM浓度的BODIPY-Cholesterol储备溶液[2]。用PBS（或其他缓冲液）稀释细胞，通过（300×g，10分钟）离心洗涤以去除多余的稀释剂，然后使用载体介质重悬细胞沉淀，达到所需浓度[2]。用BODIPY-Cholesterol标记细胞悬液（最终浓度0.5-5 μM），确保充分混合后，在37°C下孵育10-20分钟[1]。按照孵育介质中的指南，用每种适配介质稀释细胞，调整至最终浓度，并在流式细胞仪评估前孵育2小时[2]。 |
| Synonyms | BCh2 |
| molecular weight | 576.61 |
| Molecular formula | C36H51BF2N2O |
| CAS | 878557-19-8 |
| Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility | DMSO: 1 mg/mL (1.73 mM), Sonication is recommended. H2O: <0.1 mg/mL (Insoluble) |
| References | 1. Maarit Hölttä-Vuori, et al. BODIPY-cholesterol: a new tool to visualize sterol trafficking in living cells and organiss. Traffic. 2008 Nov;9(11):1839-49. 2. Daniel Wüstner, et al.Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells. Chem Phys Lipids. 2016 Jan;194:12-28. |