| Description | C11-BODIPY581/591 is a fluorescent ratio-probe of lipid oxidation . C11-BODIPY581/591 is often used for indexing lipid peroxidation and antioxidant efficacy in model membrane systems and living cells. C11-BODIPY581/591 is applied in the quantitation of ferroptosis. |
| In vitro | C11-BODIPY581/591 can easily enter membranes and exhibits good spectral separation of the nonoxidized (595 nm) and oxidized (520 nm) forms[1].Stock solutions of C11 BODIPY581/591 are made by dissolving 1 mg in 20 mM organic solvent.Cells are labeled in DMEM or PBS with 1-20 μM C11-BODIPY581/591 at 37°C, 30 min before the experiment.Alternatively, cells are incubated in DMEM with 1-10 μM preoxidized C11-BODIPY581/591. |
| Synonyms | BODIPY 581/591 C11 |
| molecular weight | 504.43 |
| Molecular formula | C30H35BF2N2O2 |
| CAS | 217075-36-0 |
| Storage | keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| References | 1. Gregor P C Drummen, et al. C11-BODIPY(581/591), an oxidation-sensitive fluorescent lipid peroxidation probe: (micro)spectroscopic characterization and validation of methodology. Free Radic Biol Med. 2002 Aug 15;33(4):473-90. |