Boc-Val-Pro-Arg-MCA hydrochloride Bioactivity,Chemical Properties,Storage & Solubility Information Pathways-PeptideDB

Description	Boc-Val-Pro-Arg-MCA hydrochloride (Boc-Val-Pro-Arg-MCA HCl) is a sensitive fluorescent substrate for the determination of trypsin-like serine proteases activity.
In vitro	按照协议指南（以下是我们推荐的操作程序。此程序仅供参考，并应根据具体需求进行相应调整），进行胰蛋白酶活性测定 [2]：首先，向含有 800 μL 50mM Tris-HCl 缓冲液 (pH 8.0) 的测试管中加入 100 μL 稀释的 Trypsin 酶。然后，加入 100 μL 50 μM 的底物 Boc-Val-Pro-Arg-MCA，以立即启动反应。接着在 55°C 的条件下孵育 10 分钟。孵育后，迅速加入 1.5 mL 的停止剂（甲醇:正丁醇:蒸馏水= 35:30:35, v/v/v），以终止反应。最后，使用荧光光谱仪在激发波长 380 nm 和发射波长 450 nm 下，测定荧光强度。
Synonyms	Boc-Val-Pro-Arg-MCA HCl
molecular weight	664.19
Molecular formula	C31H46ClN7O7
CAS	70375-24-5
Storage	store at low temperature,keep away from moisture,keep away from direct sunlight \| Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice.
References	1. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358. 2. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204.

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